rabbit polyclonal antibody against cyclin d1  (Santa Cruz Biotechnology)

Journal: Journal of translational medicine

Article Title: METTL3 regulates Ambra1 expression in an m6A-YTHDF2-dependent manner to promote mantle cell lymphoma progression.

doi: 10.1186/s12967-025-06647-4

Figure Lengend Snippet: Fig. 8 METTL3/YTHDF2/Ambra1 promotes MCL progression. (A) Representative images of tumors from each group on day 28. (B-C) Changes in tumor size and weight in the indicated groups. (D) METTL3 and Ambra1 mRNA and protein expression in the tumor tissues of the indicated groups. (E) Immu noblots showing the expression of Bcl2, BAX, cleaved caspase-3, and PARP in the tumor tissues. (F-G) Representative IHC images showing expression of cyclin D1, CDK4, and CDK6 in the tumor tissues. n = 5, *P < 0.05 vs. sh-NC + sh-NC group, #P < 0.05 vs. sh-METTL3 + sh-NC group, &P < 0.05 vs. sh-NC + sh- Ambra1 group

Article Snippet: After blocking with 5% BSA, the sections were incubated overnight with rabbit polyclonal antibodies against cyclin D1 (AWA10518, Abiowell), CDK4 (11026-1-AP, ProteinTech), and CDK6 (14052-1-AP, ProteinTech) (dilution 1:200) at 4 °C.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Exposure to b-LED Light While Exerting Antimicrobial Activity on Gram-Negative and -Positive Bacteria Promotes Transient EMT-like Changes and Growth Arrest in Keratinocytes

doi: 10.3390/ijms23031896

Figure Lengend Snippet: Exposure to b-LED causes a transient block in cell proliferation. ( A , B ) Cell viability test of HaCaT cells exposed to b-LED. HaCaT cells were pretreated with calcein AM (2 µM) and exposed to b-LED for 4 h. Cells were then analyzed 4, 24 and 48 h after beginning of exposure to b-LED. Cells were fixed and stained with DAPI. Images were acquired by fluorescence microscopy. ( A ) Calcein AM/DAPI-positive cells; ( B ) calcein AM-positive cells. Bars represent the mean ± SEM from two independent experiments. Twelve fields containing at least 30 nuclei per field were analyzed. * p < 0.05; ** p < 0.01. ( C ) Western blot showing the expression of cleaved caspase 3 in HaCaT cells exposed to b-LED for 4 h. Cells were analyzed 4, 24 and 48 h after beginning of exposure to b-LED. Western blot analysis was performed on total lysates. Tubulin was detected as a loading control. Data are representative of three independent experiments. ( D ) Cell proliferation assay of HaCaT cells exposed to b-LED. HaCaT cells were left untreated or were exposed to b-LED for 4 h and were then evaluated for cell proliferation assays 4, 24 and 48 h after beginning of exposure to b-LED using a SpectraMax 13 microplate reader. The experiment was performed in triplicate and was repeated twice. * p < 0.05; ** p < 0.01; ns, not significant. ( E – H ) HaCaT cells exposed to b-LED for 4 h and then were analyzed 4 and 24 h after beginning of exposure. ( E ) Quantitative RT-PCR expression analysis of p21 in. ( F ) Western blot expression of p21 in HaCaT cells. ( G ) quantitative RT-PCR expression analysis of Cyclin D1 in HaCaT cells. ( H ) Western blot expression of Cyclin D1 in HaCaT cells. Quantitative RT-PCR was performed on total RNA. L34 mRNA levels were used for normalization. Results are expressed in terms of fold change; bars represent the mean ± SEM of duplicate determinations in four independent experiments. * p < 0.05; *** p < 0.001 and ns, not significant. Western blot analysis was performed on total lysates. Tubulin was detected as a loading control. Data are representative of three independent experiments.

Article Snippet: Mouse monoclonal antibody against cleaved caspase 3 was from Cell Signaling Technology (Danvers, MA, USA); against E-cadherin was from BD (Franklin Lakes, NJ, USA); against tubulin was from Millipore (Merck, Kenilworth, NJ, USA); rabbit polyclonal antibodies against p21 and cyclin D1 were from Santa Cruz Biotech (Dallas, TX, USA); against SLUG was from Cell Signaling Technology; rabbit monoclonal antibody to γH2A.X (phospho S139) was from Abcam, (Cambridge, UK).

Techniques: Blocking Assay, Staining, Fluorescence, Microscopy, Western Blot, Expressing, Control, Proliferation Assay, Quantitative RT-PCR

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: The association of neuron-derived orphan receptor 1 with pulmonary vascular remodeling in COPD patients

doi: 10.2147/copd.s151820

Figure Lengend Snippet: Figure 1 Hypoxemia induced NOR1 upregulation and pulmonary vascular remodeling. Notes: Lung tissues were collected and pulmonary vascular remodeling was estimated. (A) HE staining showed the wall thickness of pulmonary vessels in patients. (B) Immunohistochemistry staining (α-SMA) showed the muscularized vessels of pulmonary vessels in patients. (C and D) Histogram showed the results of wall thickness and muscularized vessel percentage in patients. (E and F) Protein levels (SDS-PAGE and histogram) of HIF-1α, α-SMA, NOR1, and cyclin D1 in peripheral lung tissues of different patients. *P,0.01 as compared with control. Control: n=15; hypoxemia: n=11. Abbreviations: NOR1, neuron-derived orphan receptor 1; HE, hematoxylin and eosin; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.

Article Snippet: Patients and methods reagents Rabbit polyclonal antibody against GAPDH (sc-367714) human cyclin D1 (sc-718) or smooth muscle myosin heavy chain 11 (ab53219) and mouse monocloal antibody against smooth muscle actin (sc-53142) or BrdU (sc-32323) were all purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Staining, Immunohistochemistry, SDS Page, Control, Derivative Assay, Polyacrylamide Gel Electrophoresis

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: The association of neuron-derived orphan receptor 1 with pulmonary vascular remodeling in COPD patients

doi: 10.2147/copd.s151820

Figure Lengend Snippet: Figure 5 NOR1 promoted proliferation via cyclin D1. Notes: PASMCs were cultured in hypoxia (5% O2) or normoxia (21% O2) after different treatments. (A and B) Cyclin D1 protein levels (SDS-PAGE and histogram) in PASMCs after transfection with NOR1 overexpression plasmid (pNOR1). (C and D) Cyclin D1 protein levels (SDS-PAGE and histogram) in PASMCs after transfection with NOR1-siRNA or NC-siRNA. (E) BrdU incorporation in hypoxic or normoxic cells after PD0332991 (a specific inhibitor of cyclin D1-CDK4/6 complex) treatment. (F) Histogram shows the results of BrdU incorporation. (G) Results of cell counts in hypoxic or normoxic cells after PD0332991 treatment. #P,0.05 as compared with control (or normoxia), *P,0.05 as compared with NC-siRNA, &P,0.05 as compared with DMSO (n=6). Abbreviations: NC, negative control; NOR1, neuron-derived orphan receptor 1; PASMC, pulmonary arterial smooth muscle cell; SDS-PAGE, sodium dodecyl sulfate– polyacrylamide gel electrophoresis.

Article Snippet: Patients and methods reagents Rabbit polyclonal antibody against GAPDH (sc-367714) human cyclin D1 (sc-718) or smooth muscle myosin heavy chain 11 (ab53219) and mouse monocloal antibody against smooth muscle actin (sc-53142) or BrdU (sc-32323) were all purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Cell Culture, SDS Page, Transfection, Over Expression, Plasmid Preparation, BrdU Incorporation Assay, Control, Negative Control, Derivative Assay, Polyacrylamide Gel Electrophoresis